ABSTRACT
An L-asparaginase from Erwinia carotovora was successively purified by ammonium sulfate precipitation, DEAE-cellulose and Sephacryl S-200 columns. The specific activity of the L-asparaginase was increased approximately 55-fold, from 15.5 to 852 U/mg proteins. SDS-PAGE showed that the purified L-asparaginase was homogeneous and the molecular weight was about 115 kDa. The isoelectric point [pI] of the enzyme was about 5.9. Characterization of the enzyme exhibited optimum pH and temperature of 8.4 and 40°C, respectively. The purified enzyme is able to prolong its thermal stability up to 50°C. A Lineweaver-Burk analysis showed a K[m] value of 0.154 mM and V[max] of 41.67 U. The purified enzyme was rich in glycine, alanine, glutamic acid and aspartic acid
Subject(s)
Pectobacterium carotovorum/enzymology , Isoelectric Focusing/statistics & numerical data , Amino AcidsABSTRACT
Finalmente, no existen tests definitivos para EM en el laboratorio; sin embargo, los múltiples resultados anormales, producto del examen combinado del LCR pueden ser sensibles y específicos para el diagnóstico de EM. Más del 90 por ciento de los individuos con EM tendrán anormalidades en LCR. El hallazgo diagnóstico más ampliamente usado es el de síntesis de IgG localizada en LCR y la demostración de bandas oligoclonales en la zona Y por IEF o EF en gel de agarosa